Fas and Mutant Estrogen Receptor Chimeric Gene: A Novel Suicide Vector for Tamoxifen-inducible Apoptosis
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Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
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ژورنال
عنوان ژورنال: Japanese Journal of Cancer Research
سال: 1998
ISSN: 0910-5050
DOI: 10.1111/j.1349-7006.1998.tb03279.x